HPLC Column Hardware2024-07-03T08:42:51+02:00


LC Column Hardware

Biotech Fluidics supply a variety of Isolation Technologies® column hardware for HPLC and UHPLC column packing of many types of stationary phase for separations in several modes of liquid chromatography. The different types of column hardware have been designed ultra-smooth internal surfaces to ensure high separation efficiency while minimizing flow disturbances and secondary interactions that could lead to skewed peaks and low performance in liquid chromatography analysis.

The column hardware product lines we offer include inert BioVersal™ PEEK-lined stainless-steel (PLS) columns for interference-free UHPLC, IsoBar stainless-steel UHPLC column hardware, biocompatible metal-free Bio-Safe™ columns in PEEK, and modular stainless-steel column hardware. In addition, we supply traditional compression columns, and column hardware for semi-preparative separations, plus tools for column packing of the different column designs.

BioVersal™ inert PEEK-lined UHPLC column system

IDEX Health & Science introduces the NEW Isolation Technologies® PEEK-Lined Stainless Steel (PLS) Column for biocompatible applications. The PLS column combines the strength of our stainless steel UHPLC column (IsoBar) with the chemical inertness of PEEK polymer to ensure the integrity of biomolecules by minimizing unwanted surface interactions while also allowing operation under harsh solvent or pH conditions. This hardware has been designed for demanding applications within the UHPLC realm.

  • Rated to 20,000 psi (1379 bar) packing at RT/ 15,000 psi (1034 bar) operating up to 80°C
  • Removable frit assemblies
  • Excellent column durability over hundreds of injections
  • Easy-to-pack column hardware
  • Improved pressure handling capability over conventional PEEK column hardware


IsoBar™ UHPLC column system

Ultra-high pressure liquid chromatography (UHPLC) decreases the time and costs associated with analytical separations. To consistently maintain these technology advantages, you need column hardware that ensures the speed and precision of your processes. The IsoBar™ column platform delivers a leak-proof, reliable, all-metal design with high strength threaded end fittings to meet critical system operating requirements.

  • Rated up to 20000 psi (1,379 bar) making IsoBar™ the perfect solution for UHPLC technology
  • Proven format for <2 µm stationary phase
  • Features Isobore™ internal surface finish, which reduces the wall effect and significantly improves column efficiency

Bio-Safe™ PEEK column system

Bio-Safe™ columns are biocompatible and precision-machined from virgin PEEK (polyetheretherketone), a strong, inert polymer material, and are metal free for biotechnology and ion chromatography applications. PEEK columns are used when analytes are sensitive to the metals in stainless steel hardware. Proteins, peptides, and ions have this susceptibility. PEEK is also compatible with most mobile phases used in HPLC. While THF will cause PEEK to swell, this is limited to PEEK capillary tubing <1.0 mm ID. Bio-Safe™ PEEK tubing also has superior wall thickness for strength and an ultra-smooth internal surface finish (10 µin Ra) for high efficiency.

  • Completely biocompatible for optimal results in sensitive applications
  • Available with PEEK or Titanium frits (several size options available)
  • Convenient, strong threaded design that is stable at pressures up to 8,000 psi (552 bar)
  • High efficiency connections and surface finish for optimum performance
  • Bio-Safe packing adapters are available for inner diameters of 2.1 mm and 4.6 mm

Modular HPLC column system

Modular hardware guarantees a high-pressure seal between the tubing and end fitting, as well as a proper seating of the frit. The frit is contained in a cap that slides over the column, forming a leak-free and gap-free seal. All stainless-steel modular columns use our exclusive Isobore™ internal surface finish. Modular column hardware systems include the following components: one 316 stainless steel column body, two one-piece end fittings and two frit caps containing 316 stainless steel frits in a PEEK™ cap. In addition, Modular systems are available in custom IDs and lengths — many sizes are available. Isobore-grade surface finish reduces drag at the column wall and im[1]proves column efficiency and performance by providing an extremely smooth, flat, and clean surface; it also features a surface finish with typical values around 8 µin.

  • Operates at pressures up to 15,000 psi (1034 bar)
  • Precision machined from high purity LC grade 316 stainless steel

Classic compression column system

  • Operates at pressures up to 10,000 psi (689 bar)
  • Multiple port configurations make columns easy to use with any system
  • Leak-free frits improve system efficiency
  • Isobore™ surface finish reduces drag at the column wall and improves column efficiency and performance by providing an extremely smooth, flat, and clean surface; it also features a surface finish with typical values around 8 µin.

Any questions?

If you have any inquiries about our products and services, we’re here to help.

Phone: +46 300 56 91 80
Email: info@biotechfluidics.com

News & insights about HPLC column hardware

IDEX Catalogue update 2024

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As the Global Premium Distributor for IDEX, Biotech Fluidics can present an updated IDEX catalogue with flow components, separation tools, and fluid management products. The catalogue has been thoroughly

New biocompatible PEEK-lined stainless steel column hardware

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IDEX Health & Science introduces a new PEEK-Lined Stainless Steel (PLS) column hardware for biocompatible applications. The PLS column hardware combines the strength of our stainless steel UHPLC column

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