Inline filters
Biotech Fluidics provide inline filters to protect sensitive equipment parts at a range of different conditions. We offer various types of inline filters for flow rates from a few microlitres up to several millilitres per minute. Some of these filters are biocompatible by offering a PEEK-based design with a metal-free flow path, whereas other are based on stainless-steel.
Note that although the design of the bodies for precolumn filters and inline filters look similar and both are used for HPLC applications, their intended use is rather different. Precolumn filters, by definition, are typically placed in a volume-sensitive area immediately preceding the HPLC column. These filters usually feature smaller thru-holes and smaller frit diameters to minimize effect on chromatographic peak shapes. In contrast, inline filters are placed where the internal volume is not as critical and where longer life and less fluid restriction is more important.
Several variants available:
- Analytical inline filters
- Analytical precolumn filters
- Semi-prep inline filters
- Inline MicroFilters
- Mini MicroFilters
- Precolumn MicroFilters
Analytical inline filters
Standard analytical inline filters
Our Inline Filters are specially engineered for inline filtration. It is specifically designed to help prevent particulate contamination from clogging sensitive equipment. It is ideally suited for placement along the flow path line between the pump and injection valve/autosampler. We offer a variety of porosities for your application.
- For 1/16” OD tubing
- For Standard HPLC (6,000 psi/414 bar)
- Replacement frits available
- Help prevent particulate contamination from clogging sensitive equipment
- Ideally suited for placement along the flow path line between the pump and injection valve/autosampler
Biocompatible analytical inline filters
Our A-430 and A-431 Inline Filters consist of a stainless steel body and two PEEK end fittings. Maximum recommended flow rate is 25 mL/min for the A-430 Filter and 10 mL/min for the A-431 Filter. And, you get the added benefit of biocompatibility since all wetted surfaces are PEEK. When you need to replace the frit, simply dispose of the end fitting that contains the frit and replace it with a new one.
- 0.5 µm and 2 µm versions available
- Features 100% PEEK flow path
| Prod. No.* | Description | Porosity | For Tubing Size | Port | Includes | Swept Volume | Pressure Rating |
|---|---|---|---|---|---|---|---|
| A-313 | Inline Filter Assembly | 20 µm | 1/16” OD | 10-32 Coned | A-224 | 12.3 µL | 6,000 psi (414 bar) |
| A-314 | Inline Filter Assembly | 2 µm | 1/16” OD | 10-32 Coned | A-100 | 4 µL | 6,000 psi (414 bar) |
| A-100 | Replacement Frit, Stainless Steel, 1 ea | 2 µm | - | - | - | 1.4 µL | - |
| A-224 | Replacement Frit, Stainless Steel, 1 ea | 20 µm | - | - | - | 9.7 µL | - |
| A-430 | Biocompatible Inline Filter Assembly | 2 µm | 1/16” OD | 10-32 Coned | A-429 | 7.1 µL | 6,000 psi (414 bar) |
| A-431 | Biocompatible Inline Filter Assembly | 0.5 µm | 1/16” OD | 10-32 Coned | A-428 | 5.9 µL | 6,000 psi (414 bar) |
| A-428 | PEEK Filter End Fittings, Black PEEK body | 0.5 µm | - | - | - | 5.7 µL | - |
| A-429 | PEEK Filter End Fittings, Black PEEK body | 2 µm | - | - | - | 6.9 µL | - |
Detailed information about the replacement filters are available at the webpages for Stainless-steel frits and PEEK frits, respectively.
Analytical precolumn filters
Standard precolumn filters
Our economical Precolumn Filters offer secure protection for analytical columns in HPLC. We offer traditional versions that can successfully connect tubing on both sides and our direct-connect versions attach to the inlet port of most standard columns. All versions feature a 10-32 coned ports for 1/16” OD tubing. These are designed to protect columns by filtering out particulate matter originating from the sample or from rotor seal wear.
- Economical protection for larger columns and injections
- Traditional versions connect tubing on both sides
- Direct-connect versions attach to the inlet port of most standard columns
- All versions feature 10-32 coned ports for 1/16” OD tubing
Biocompatible precolumn filters
Biocompatible Precolumn Filters have 0.020” (0.50 mm) diameter thru-holes and 8° distribution cones for minimal band spreading and mixing. The bodies of these filters are manufactured from biocompatible PEEK polymer and are pressure rated to 5,000 psi (345 bar). These filters are designed for use with 1/16” OD tubing, which can be connected to these filters using standard Fingertight fittings.
- Pre-assembled with either 0.5 µm or 2 µm porosity frits
- Great column protection
- Feature PEEK bodies and PCTFE-surrounded PEEK frits
| Prod. No.* | Description | Porosity | For Tubing Size | Port | Includes | Swept Volume | Pressure Rating |
|---|---|---|---|---|---|---|---|
| A-315 | Precolumn Filter Assembly | 2 µm | 1/16” OD | 10-32 Coned | A-101 | 1.4 µL | 6,000 psi (414 bar) |
| A-318 | Precolumn Filter Assembly | 0.5 µm | 1/16” OD | 10-32 Coned | A-102 | 0.84 µL | 6,000 psi (414 bar) |
| A-101 | Replacement Frit, Stainless Steel | 2 µm | - | - | - | 0.74 µL | - |
| A-102 | Replacement Frit, Stainless Steel | 0.5 µm | - | - | - | 0.61 µL | - |
| A-355 | Biocompatible Precolumn Filter Assembly | 2 µm | 1/16” OD | 10-32 Coned | A-700 | 1.4 µL | 5,000 psi (345 bar) |
| A-356 | Biocompatible Precolumn Filter Assembly | 0.5 µm | 1/16” OD | 10-32 Coned | A-701 | 1.3 µL | 5,000 psi (345 bar) |
| A-700 | Replacement Frit, PEEK Polymer | 2 µm | - | - | - | 0.74 µL | - |
| A-701 | Replacement Frit, PEEK Polymer | 0.5 µm | - | - | - | 0.61 µL | - |
Detailed information about the replacement filters are available at the webpages for Stainless-steel frits and PEEK frits, respectively.
Semi-preparative inline filters
Standard semi-prep inline filters
Our Semi-Prep Filter Assemblies are meticulously designed for high flow applications. They are SFC and HPLC compatible and adds only 50 psi (3 bar) additional back pressure. They have a porosity of 10 µm and includes a stainless steel frit (A-331). Alternative filter porosities are available.
- Designed for high-flow applications
- Economical protection for larger columns and injections
- SFC and HPLC compatible
Biocompatible semi-prep inline filters
Biocompatible Semi-Prep Filters consist of a stainless steel body, two PEEK end fittings, and a separate PEEK frit. These filters are ideal for many higher flow analytical, semi-prep and preparative applications. Best of all, if the filter becomes clogged, simply unscrew the assembly, remove the frit and replace it. The frits are interchangeable.
- Versions for 1/16”, 1/8”, & 3/16” OD tubing
- 100% PEEK flow path
| Prod. No.* | Description | Porosity | For Tubing Size | Port | Includes | Swept Volume | Pressure Rating |
|---|---|---|---|---|---|---|---|
| A-330 | Standard Semi-Prep Filter Assembly | 10 µm | 1/16" OD | 10-32 Coned | A-331 | 223 µL | 7,500 psi (517 bar) |
| A-360 | Standard Semi-Prep Filter Assembly | 10 µm | 1/16", 1/8", or 3/16" OD | 5/16-24 Flat Bottom | A-331 | 235 µL | 3,500 psi (207 bar) |
| A-331 | Stainless Steel Frits, Natural ETFE ring | 10 µm | - | - | - | 142 µL | - |
| A-332 | Stainless Steel Frits, Natural ETFE ring | 2 µm | - | - | - | 122 µL | - |
| A-337 | Stainless Steel Frits, Natural ETFE ring | 20 µm | - | - | - | 152 µL | - |
| 1020-05 | Iso-Prep 21.2 mm Filter Holder | 0.5 µm | 1/16" OD | 10-32 Coned | 7031-05 | 203 µL | 8,000 psi (552 bar) |
| 1020-20 | Iso-Prep 21.2 mm Filter Holder | 2 µm | 1/16" OD | 10-32 Coned | 7031-20 | 196 µL | 8,000 psi (552 bar) |
| 7031-05 | Iso-Prep 21.2 mm Replacement Filter | 0.5 µm | - | - | - | 122 µL | 8,000 psi (552 bar) |
| 7031-20 | Iso-Prep 21.2 mm Replacement Filter | 2 µm | - | - | - | 115 µL | 8,000 psi (552 bar) |
| A-410 | Biocompatible Semi-Prep Filter Assembly | 2 µm | 1/16" OD | 10-32 Coned | OC-802 | 89 µL | 6,000 psi (414 bar) |
| A-411 | Biocompatible Semi-Prep Filter Assembly | 10 µm | 1/16" OD | 10-32 Coned | OC-803 | 103 µL | 6,000 psi (414 bar) |
| A-510 | Biocompatible Semi-Prep Filter Assembly | 5 µm | 1/16", 1/8", or 3/16" OD | 5/16-24 Flat Bottom | OC-805 | 89 µL | 500 psi (34 bar) |
| OC-802 | PEEK Frit, Green PCTFE ring | 2 µm | - | - | - | 46 µL | - |
| OC-803 | PEEK Frit, Natural PCTFE ring | 10 µm | - | - | - | 57 µL | - |
| OC-805 | PEEK Frit, Natural PCTFE ring | 5 µm | - | - | - | 50 µL | - |
Detailed information about the replacement filters are available at the webpages for Stainless-steel frits and PEEK frits, respectively.
Inline MicroFilters
Our Inline MicroFilters protect your column from particles originating in the mobile phase or sample, or from pump seal and sample injection valve wear. These filters have a 0.006” (150 µm) thru-hole. Choose the M-520 with a 0.5 µm 100% PEEK frit to connect to capillary tubing using the MicroTight® tubing sleeves. You may also directly connect 1/32” OD tubing using the M-525 which contains a 0.5 µm PEEK frit.
- 100% biocompatible PEEK polymer option available
- Miniscule 240 nL void volume
- Two versions: direct connect 1/32” OD tubing or use MicroTight® tubing sleeves for 70–520 µm OD capillary tubing
| Prod. No.* | Description | Porosity | For Tubing Size | Port | Includes | Swept Volume | Pressure Rating |
|---|---|---|---|---|---|---|---|
| M-520 | Inline MicroFilter Assembly, PEEK Frit | 0.5 µm | MicroTight Tubing Sleeve | 6-32 Coned | M-120 (5), F-125 (2) | 240 nL | 4,000 psi (276 bar) |
| M-525 | Inline MicroFilter Assembly, PEEK Frit | 0.5 µm | 1/32” OD | 6-32 Coned | M-140 (5), F-126S (2) | 240 nL | 4,000 psi (276 bar) |
| M-120 | End-Fittings, Black, with PEEK Frit | 0.5 µm | MicroTight Tubing Sleeve | 6-32 Coned | - | 216 nL | - |
| M-140 | End-Fittings, Natural, with PEEK Frit | 0.5 µm | 1/32” OD | 6-32 Coned | - | 216 nL | - |
Mini MicroFilters
Our Mini MicroFilter Assemblies filter effectively with internal volumes low enough to ensure reliable chromatographic results — even at nanoliter per minute flow rates! Internal volumes of these encapsulated filters are as low as 85 nL with the micro-screen and 10 nL to 22 nL with the frit disc option.
- Total volume as low as 10 nL
- Conductive version for CEC and mass spectrometry applications
| Prod. No.* | Description | Porosity | Frit Type | For use with | Includes | Swept Volume | Pressure Rating |
|---|---|---|---|---|---|---|---|
| M-547 | Mini MicroFilter Assembly | 1 µm | SST Frit | 1/32” (790 µm) OD tubing | M-133 (5), F-112 (2), P-416 (2) | 22 nL | 4,000 psi (276 bar) |
| M-121 | Filter Capsule, PEEK, 2 ea | 1 µm | SST Screen | M-530 and M-531 | - | 85 nL | - |
| M-125 | NanoFilter Capsule, PEEK, 2 ea | 1 µm | SST Frit | M-537 and M-538 | - | 10 nL | - |
| M-131 | Filter Capsule, PEEK, 2 ea | 1 µm | SST Screen | M-543 | - | 85 nL | - |
| M-133 | NanoFilter Capsule, PEEK, 2 ea | 1 µm | SST Frit | M-547 and M-548 | - | 10 nL | - |
| M-128 | Conductive NanoFilter Capsule, SST/PEEK, 2 ea | 1 µm | SST Frit | M-534 | - | 10 nL | - |
Precolumn MicroFilters
The Precolumn MicroFilters directly connect into your microbore or analytical column. Total theoretical void volume is only 0.5 µL (includes frit volume) and the PEEK tubing used in the assembly of these units has a 0.005” (125 µm) ID, virtually eliminating any mixing of the sample with the mobile phase.
- Direct connects to columns with 10-32 threads
- Total void volume of 0.5 µL
- Two versions: direct connect 1/16” OD tubing or use MicroTight® tubing sleeves for 70–520 µm OD capillary tubing
| Prod. No.* | Description | Porosity | For Tubing Size | Port | Includes | Swept Volume | Pressure Rating |
|---|---|---|---|---|---|---|---|
| M-500 | Precolumn MicroFilter Assembly, SST Frit | 0.5 µm | MicroTight Tubing Sleeve | 10-32 Coned | C-425 (5), F-172, P-416 | 0.5 µL | 4,000 psi (276 bar) |
| M-510 | Precolumn MicroFilter Assembly, PEEK Frit | 0.5 µm | MicroTight Tubing Sleeve | 10-32 Coned | A-735 (5), F-172, P-416 | 0.5 µL | 4,000 psi (276 bar) |
| M-550 | Precolumn MicroFilter Assembly, SST Frit | 0.5 µm | 1/16" OD | 10-32 Coned | C-425 (5), F-132, P-416 | 0.5 µL | 4,000 psi (276 bar) |
| M-555-1 | Precolumn MicroFilter Assembly, SST Frit | 2 µm | 1/16" OD | 10-32 Coned | C-420 (5), F-132, P-416 | 0.5 µL | 4,000 psi (276 bar) |
| M-560 | Precolumn MicroFilter Assembly, PEEK Frit | 0.5 µm | 1/16" OD | 10-32 Coned | A-735 (5), F-132, P-416 | 0.5 µL | 4,000 psi (276 bar) |
| A-735 | PEEK Frits, 0.045” x 0.031” x 0.192” | 0.5 µm | - | - | - | 216 nL | - |
| C-420 | SST Frits, 0.038” x 0.028” x 0.192” | 2 µm | - | - | - | 101 nL | - |
| C-425 | SST Frits, 0.038” x 0.028” x 0.192” | 0.5 µm | - | - | - | 101 nL | - |
Detailed information about the replacement filters are available at the webpages for Stainless-steel frits and PEEK frits, respectively.
